Data#
What exists#
| Dataset | Format | Status |
|---|---|---|
| SDS-PAGE and clear-native gel images | TIFF / PNG from gel imager | Collected across May–September |
| Anti-His immunoblots | TIFF / PNG | Collected |
| SEC chromatograms | UNICORN export | Collected, multiple runs per construct |
| DLS distributions | Instrument export | Collected |
| A280 step-by-step purification tables | Spreadsheet | Collected |
| Sanger and whole-plasmid sequencing | AB1 / FASTA, aligned in Benchling | Collected |
| Growth curves | — | Not yet — awaits the selection screen |
| Selection screen dose–response | — | Not yet |
| Evolution campaign sequencing | — | Not yet |
Purification step accounting#
The most useful thing produced this season is not a single measurement but the per-step aliquot series: an aliquot retained at every stage of every purification, with A280 and a gel lane for each. That is what turns "the protein disappeared" into "the protein is in the pellet after lysis", which is a debuggable statement.
Sequencing#
All constructs were verified by Sanger sequencing of assembly junctions and, where it mattered, by whole-plasmid sequencing. Alignments are maintained in Benchling.
Negative sequencing results are retained deliberately — the clones that came back as empty backbone or as an unrelated insert are part of the record of how long the assemblies took to get right.
Availability#
Raw data will be deposited alongside the report. Sequences of all constructs will be provided as supplementary information; plasmid maps are maintained in Benchling.
Not yet populated
This page lists what has been collected, not links to it. Deposition happens with the report submission.