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The experimental objective in March: To construct tryptophan biosensors/plasmids, knockout/mutate genes, and verify functions for screening high-tryptophan strains.
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The experimental objective in April: Construct and optimize tryptophan biosensors, verify gene knockout effects, and complete CysE knockout and verification to aid strain research.
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The experimental objective in May: Construct and validate E. coli cysteine auxotroph biosensor; knockout 8 genes via electroporation to verify plasmid efficacy.
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The experimental objective in June: Construct four new plasmids, test sensor response to tryptophan, and evaluate tryptophan-to-melatonin pathways via plasmid assembly.
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The experimental objective in July: Construct and validate shikimic acid sensor, test tryptophan in knockout strains, and evolve BW∆trpR∆tnaAB to boost yield.
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The experimental objective in August: Overexpressing individual genes in the shikimic acid pathway to explore whether it can increase tryptophan yield.The yield of the whole-genome mutant strain was tested by fermentation. The metabolic pathway was further optimized, and glnA and yddG genes were overexpressed to test the level of tryptophan synthesis.
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Organize all experimental data during the period and begin writing the final report and creating the wiki
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