Member: H.T.Q，Z.M.L，L.Y，Y.Y.X，L.Y.X

Experiment:

"(1)Confect LB medium of 500 ml (solid, liquid)

(2)Transformation : AP Breed at constant temperature of 37℃ for 12 h."

Result:

LB solid medium is supplemented with the ineffective antibiotic Amp.

Member: Y.Y.X

(1)Design three pairs of primers to prepare for the construction of recombinant plasmid AP-T7-P.