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DNA sequences used in this study
| Names | Sequences |
|---|---|
| WT-i-F | ctttgcgatgctgagcattggcaaagtg |
| WT-i-R | cagaatcaccaccggtttaatcagatctttcg |
| P415-v-R | ccaatgctcagcatcgcaaagcctttcatttttttcccgggggatccactagttc |
| P415-v-F | gattaaaccggtggtgattctggcgtaatctcttctcgagtcatgtaattagttatgtcacg |
| pEvolvR-i-R-2 | gtgagcctggtcccagttttcaccagaaccaac |
| pEvolvR-i-F-2 | ggacaagaagtattctatcggactggctatcgggactaatagc |
| scaffold-409-v-R | aaagaaattccgctggaagctcctccatagagaacgtatcgatcatttatctttc |
| scaffold-170-v-R | gaatcatgttatggcgttcgtcctccatagagaacgtatcgatcatttatctttc |
| scaffold-409-v-F | gcttccagcggaatttctttgttttagagctagaaatagcaagttaaaataaggctagtc |
| scaffold-170-v-F | cgaacgccataacatgattcgttttagagctagaaatagcaagttaaaataaggctagtc |
| TBSADH-S-294295-R | taaaaccattaaaggcggcggtatcccgggcggccgcctgc |
| TBSADH-S-294295-F | gcaggcggccgcccgggataccgccgcctttaatgg |
| TBSADH-R-294295-F | gcccggggagacgccgcctttaatggttttatgcgccatgccgc |
| TBSADH-R-294295-R | gcgcataaaaccattaaaggcggcgtctccccgggcggccg |
| TBSADH-R-110-R | gcatagcggcggcatgctggcgggctggaaatttagcaacg |
| TBSADH-R-110-F | ctttcacgttgctaaatttccagcccgccagcatgccgc |
| GDH-v-R | gacttttccttttaaatccggatacatgccctgaaaatacaggttttcatgatgatgatgatg |
| GDH-v-F | cttcattccaggcaggccgcggttaaacaaagcccgaaaggaagctgagttg |
| GDH-i-R | gcggcctgcctggaatgaagggtattgtgtc |
| GDH-i-F | ccggatttaaaaggaaaagtcgtcgctatcacaggag |
| S-Tbs294G295I-F | ccgcccgggataccgccgcctttaatggttttatgcgcc |
| S-Tbs294G295I-R | ttaaaggcggcggtatcccgggcggccgcctgcgcatggaac |
| S-Tbs294G295d-R | ccgcccgggtcaccgccgcctttaatggttttatgcgcc |
| S-Tbs294G295d-f | ttaaaggcggcggtgacccgggcggccgcctgcgcatggaac |
| S-Tbs294G295V-f | ttaaaggcggcggtgtcccgggcggccgcctgcgcatggaac |
| S-Tbs294G295V-R | ccgcccgggacaccgccgcctttaatggttttatgcgcc |
| TbsADH-S-2257 | catcatcatcatcatcatgaaaacctgtattttcagggc atgaaaggctttgcgatgctgagcattggcaaagtgggc tggattgaaaaagaaaaaccggcgccgggcccgtttgat gcgattgtgcgcccgctggcggtggcgccgtgcaccagc gatattcataccgtgtttgaaggcgcgattggcgaacgc cataacatgattctgggccatgaagcggtgggcgaagtg gtggaagtgggcagcgaagtgaaagattttaaaccgggc gatcgcgtggtggtgccggcgctgaccccggattggcgc accagcgaagtgcagcgcggctatcatcagcatagcggc ggcatgctggcgggccagaaatttagcaacgtgaaagat ggcgtgtttggcgaattttttcatgtgaacgatgcggat atgaacctggcgcatctgccgaaagaaattccgctggaa gcggcggtgatgattccggatatgatgaccaccggcttt catggcgcggaactggcggatattgaactgggcgcgacc gtggcggtgctgggcattggcccggtgggcctgatggcg gtggcgggcgcgaaactgcgcggcgcgggccgcattatt gcggtgggcagccgcccggtgtgcgtggatgcggcgaaa tattatggcgcgaccgatattgtgaactataaagatggc ccgattgaaagccagattatgaacctgaccgaaggcaaa ggcgtggatgcggcgattattgcgggcggcaacgcggat attatggcgaccgcggtgaaaattgtgaaaccgggcggc accattgcgaacgtgaactattttggcgaaggcgaagtg ctgccggtgccgcgcctggaatggggctgcggcatggcg cataaaaccattaaaggcggccagtgcccgggcggccgc ctgcgcatggaacgcctgattgatctggtgttttataaa cgcgtggatccgagcaaactggtgacccatgtgtttcgc |
| TbsADH-R-2074 | catcatcatcatcatcatgaaaacctgtattttcagggc atgaaaggctttgcgatgctgagcattggcaaagtgggc tggattgaaaaagaaaaaccggcgccgggcccgtttgat gcgattgtgcgcccgctggcggtggcgccgtgcaccagc gatattcataccgtgtttgaaggcgcgattggcgaacgc cataacatgattctgggccatgaagcggtgggcgaagtg gtggaagtgggcagcgaagtgaaagattttaaaccgggc gatcgcgtggtggtgccggcgaataccccggattggcgc accagcgaagtgcagcgcggctatcatcagcatagcggc ggcatgctggcgggctggaaatttagcaacgtgaaagat ggcgtgtttggcgaattttttcatgtgaacgatgcggat atgaacctggcgcatctgccgaaagaaattccgctggaa gcggcggtgatgattccggatatgatgaccaccggcttt catggcgcggaactggcggatattgaactgggcgcgacc gtggcggtgctgggcattggcccggtgggcctgatggcg gtggcgggcgcgaaactgcgcggcgcgggccgcattatt gcggtgggcagccgcccggtgtgcgtggatgcggcgaaa tattatggcgcgaccgatattgtgaactataaagatggc ccgattgaaagccagattatgaacctgaccgaaggcaaa ggcgtggatgcggcgattattgcgggcggcaacgcggat attatggcgaccgcggtgaaaattgtgaaaccgggcggc accattgcgaacgtgaactattttggcgaaggcgaagtg ctgccggtgccgcgcctggaatggggctgcggcatggcg cataaaaccattaaaggcggcctgaatccgggcggccgc ctgcgcatggaacgcctgattgatctggtgttttataaa cgcgtggatccgagcaaactggtgacccatgtgtttcgc ggctttgataacattgaaaaagcgtttatgctgatgaaa gataaaccgaaagatctgattaaaccggtggtgattctggcgtaa |
| TbsADH-WT | catcatcatcatcatcatgaaaacctgtattttcagggc atgaaaggctttgcgatgctgagcattggcaaagtgggc tggattgaaaaagaaaaaccggcgccgggcccgtttgat gcgattgtgcgcccgctggcggtggcgccgtgcaccagc gatattcataccgtgtttgaaggcgcgattggcgaacgc cataacatgattctgggccatgaagcggtgggcgaagtg gtggaagtgggcagcgaagtgaaagattttaaaccgggc gatcgcgtggtggtgccggcgattaccccggattggcgc accagcgaagtgcagcgcggctatcatcagcatagcggc ggcatgctggcgggctggaaatttagcaacgtgaaagat ggcgtgtttggcgaattttttcatgtgaacgatgcggat atgaacctggcgcatctgccgaaagaaattccgctggaa gcggcggtgatgattccggatatgatgaccaccggcttt catggcgcggaactggcggatattgaactgggcgcgacc gtggcggtgctgggcattggcccggtgggcctgatggcg gtggcgggcgcgaaactgcgcggcgcgggccgcattatt gcggtgggcagccgcccggtgtgcgtggatgcggcgaaa tattatggcgcgaccgatattgtgaactataaagatggc ccgattgaaagccagattatgaacctgaccgaaggcaaab ggcgtggatgcggcgattattgcgggcggcaacgcggata ttatggcgaccgcggtgaaaattgtgaaaccgggcggcac cattgcgaacgtgaactattttggcgaaggcgaagtgctg ccggtgccgcgcctggaatggggctgcggcatggcgcata aaaccattaaaggcggcctgtgcccgggcggccgcctgcg catggaacgcctgattgatctggtgttttataaacgcgtg gatccgagcaaactggtgacccatgtgtttcgcggctttg ataacattgaaaaagcgtttatgctgatgaaagataaacc gaaagatctgattaaaccggtggtgattctggcgtaa |
Plasmid Map
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