SDS-PAGE

1. Crushing bacteria: Take 1 mL of induced and uninduced bacterial solution respectively, centrifuge at 5000 rpm for 5 min, discard the supernatant, and add 100 μL ddH2O resuspension; Or select 100 μL the supernatant of induced and uninduced cells was broken.

2. Denaturation: Add SDS loading to 1X, mix well, and heat at 95 °C for 7-10 min.
▲ The bacterial solution sample needs to be centrifuged at 12000 rpm for 10 minutes.

3. Sample loading: The supernatant shall be loaded in the manner of uninduced, induced and uninduced.

4. Wash the remaining SDS: When time is over, immerse the gel in clean water and heat it for 2 min at high temperature, change the water, repeat again, and drain the water.

5. Dyeing: Immerse the dye (such as Coomassie brilliant blue) over the gel, heat it for 2min at medium high heat, and recover the dye.

Decolorization: Wash the dyed glue repeatedly with clean water until the background is nearly transparent, and observe it with a photometer under a white background.
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