1. Crushing bacteria: Take 1 mL of induced and uninduced bacterial solution respectively, centrifuge at 5000 rpm for 5 min, discard the supernatant, and add 100 μL ddH2O resuspension; Or select 100 μL the supernatant of induced and uninduced cells was broken.
2. Denaturation: Add SDS loading to 1X, mix well, and heat at 95 °C for 7-10 min.
▲ The bacterial solution sample needs to be centrifuged at 12000 rpm for 10 minutes.
3. Sample loading: The supernatant shall be loaded in the manner of uninduced, induced and uninduced.
4. Wash the remaining SDS: When time is over, immerse the gel in clean water and heat it for 2 min at high temperature, change the water, repeat again, and drain the water.
5. Dyeing: Immerse the dye (such as Coomassie brilliant blue) over the gel, heat it for 2min at medium high heat, and recover the dye.
Decolorization: Wash the dyed glue repeatedly with clean water until the background is nearly transparent, and observe it with a photometer under a white background.