In background research, we shared with them the contact information of the dyeing factory we had visited; we also shared with each other the data from the questionnaires on the public's attitude towards bioprocessing and the social acceptance of the project.

For wet lab, we both conducted experiments on directed evolution. We exchanged our library building and screening methods, and evaluated each other and gave suggestions for optimization.

Our screening method is helpful for SCUT-China to achieve high enrichment of positive clones, compared to their mutant library which contains both positive and negative clones. SCUT-China said that they could consider designing gene routes to link PTS positive clones and thermusaquaticus synthesis in bacteria. However, due to time constraints, it is hoped that this can be continued in the future.

SCUT-China also suggested that we should try to visualize the positive clones and try visual characterization with the yield of Tyrian Purple to further validate and screen more positive clones in the future.

We shared the detailed conditions and protocals of preliminary screening method on plate with them to design their experiments.

For education, we sent each other some pigment products to complete a beautiful downstream artwork: a drip gel handicraft. We hope more people would learn about the magic and beauty of synthetic biology.

Besides, we shared our teaching program of tie-dye to SCUT-China, which gave them good inspiration and reference towards the high school to start an enriching education activity.