Prologue
Safety is one of the most noteworthy issues in experiments. Having a good experimental environment is a prerequisite for making the ideal experimental results. Therefore, our team, NEFU_China, implements several laboratory criteria to ensure the experimental safety and has always been practicing.
Safety of laboratory
All of our experiments are performed in BSL1 laboratory. Our laboratory is equipped with emergency medicine kits, emergency showers, eye baths and other emergency measures. We have daily safety checks and carry out regular comprehensive inspections. For waste treatment, we have a complete process. The waste will be disposed of or recycled according to corresponding regulations. For recyclables, we perform high temperature steam sterilization. Our lab has only a small stockpile of contaminants. Since we are exposed to dangerous chemicals, such as staining the gel with GoldView and protein electrophoresis with SDS and other substances, there are separate contaminated areas in the laboratory for contamination experiments.
Safety of project
We use E. coli DH5α and E. coli BL21(DE3) which are on the RRform white list. However, combined with the experimental design, since E. coli contains the cytosine deaminase(codA) gene which can produce CDase to affect the role of the dual selection system, we knocked out the codA gene by pCas9 on the basis of E. coli BW25113 and obtained the E. coli BWΔcodA for the construction of the mutant library.
Safety of human practice
Before entering the laboratory, all members learned and mastered the microbiological experiment operation methods and precautions with the help of the laboratory seniors, and successfully passed the laboratory safety test. During the experiment, all members are required to wear goggles, gloves and lab coats to operate. During the epidemic prevention and control period, we ensure that masks are worn throughout the test period.
