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24h culture OD600 readings and CFU serial dilution plating

Wet lab Both projects Confirmed

Contributors: Tank, Chester

OD600 of the 24 h cultures from 13 August:

CultureOD600
1a — glucose + beta-glucosidase1.88
1b — glucose + beta-glucosidase1.86
2a — glucose + CxnA1.76
2b — glucose + CxnA1.88
3a — cellobiose + CxnA0.250
3b — cellobiose + CxnA0.210
4a — cellobiose + beta-glucosidase0.530
4b — cellobiose + beta-glucosidase0.530

Both glucose conditions reached comparable, high density regardless of strain; both cellobiose conditions ended up substantially lower, with CxnA (3a/3b) even lower than beta-glucosidase (4a/4b).

Serial dilutions (10⁻³, 10⁻⁴, 10⁻⁵) of each culture were plated on LB + 40 µg/mL chloramphenicol for CFU counting the following incubation period.