Protocol
Ligation
This protocol is used to ligate two or more pieces of DNA together. For more detailed information, please click on the link.
Open PDF ↗Nucleic acid and protein electrophoresis
This protocol covers two types of electrophoresis used to detect target DNA or protein. For more detailed information, please click on the link.
Open PDF ↗DNA gel extraction
This protocol is used to recover the target DNA fragment from agarose gel. For more detailed information, please click on the link.
Open PDF ↗Polymerase chain reaction
This protocol is used to amplify target DNA fragments for plasmid construction or other applications. For more detailed information, please click on the link.
Open PDF ↗Enzyme digestion verification
This protocol is used to verify the enzyme digestion result of a DNA sample. For more detailed information, please click on the link.
Open PDF ↗PCR product purification
This protocol is used to purify PCR products (when a single band is observed by gel electrophoresis). For more detailed information, please click on the link.
Open PDF ↗Plasmid DNA extraction
This protocol is used to extract plasmid DNA from E. coli. For more detailed information, please click on the link.
Open PDF ↗Restriction endonuclease reactions
This protocol describes restriction endonuclease reactions using NEB restriction enzymes. For more detailed information, please click on the link.
Open PDF ↗Preparation of electrocompetent cells
This protocol is used to prepare electrocompetent cells with high transformation efficiency. For more detailed information, please click on the link.
Open PDF ↗Transformation
This protocol is used to introduce plasmid DNA into competent cells via chemical treatment or electroporation. For more detailed information, please click on the link.
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