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X-gluc read — colonies picked for sequencing, miniprep list drawn up

Wet lab Both projects Confirmed

Contributors: Esther, Tank

X-gluc — interesting colonies to sequence/test (p. 163)

  • β-glucosidase + control, flagged for sequencing
  • CO: 12
  • T: 4, 15, (a third number here is scribbled out in the original)

Miniprep planned for tomorrow

  • β-glucosidase
  • CO2
  • CO4
  • CO7
  • CO12
  • T4
  • T15 (the last digit of this label is overwritten in the original — transcribed as written)
  • T14

Plus whole-plasmid sequencing: “8 sequences to send off, +1” (as written).

This list’s 8th named sample is the β-glucosidase control, not an unmutated codon-optimised construct — worth checking against the sequencing writeup on the Results page, which describes the 8th sample sent as the parental codon-optimised construct rather than β-glucosidase. The “+1” in the notebook isn’t resolved here as to what it refers to.

Page 164 (the page that would follow this one) wasn’t in the set of photos this entry was transcribed from — a known gap, not an omission on this page’s part.