Contributors: Esther, Tank
Colony naming fixed (pp. 162–163)
From this point, colonies are numbered 1–15 within each library: CO (codon-optimised CxnA) and T (native-sequence CxnA), plus three single control colonies — CxnA, CxnA-His, and β-glucosidase.
Nanodrop (OD) of colonies + controls
Red annotation on the page: “11:30–16:30” (the reading window).
| Colony | T | CO |
|---|---|---|
| 1 | 0.958 | 1.052 |
| 2 | 0.222 | 1.030 |
| 3 | 0.166 | 1.092 |
| 4 | 0.369 | 0.833 |
| 5 | 0.004 | 0.423 |
| 6 | 0.294 [?] | 0.378 |
| 7 | 1.508 [?] | 0.371 |
| 8 | 0.143 | 0.684 |
| 9 | 0.433 | 1.298 |
| 10 | 0.727 | 0.482 |
| 11 | 0.191 (flagged red) | 0.850 |
| 12 | 0.349 | 0.344 |
| 13 | 0.666 | 0.356 |
| 14 | 0.893 | 0.616 |
| 15 | 0.359 | 0.495 |
| 16 | – | not recorded |
A red note at the bottom of the page reads (partly illegible): “…11/12 are not actual data.” The exact scope of this caveat isn’t clear from the transcription — it may mean colonies 11 and/or 12 in this particular OD table should be disregarded. Flagged here rather than guessed at; the original page should be checked before relying on either row.
Controls
| Control | OD |
|---|---|
| CxnA | 0.911 |
| CxnA-His | 0.257 |
| β-glucosidase | 0.546 |