Contributors: Tank, Esther
Checking the codon-optimised epPCR plates (p. 161)
Codon-optimised CxnA epPCR plates checked: the normal (insert-containing) plate showed growth, the control showed no growth. Photos taken on Esther’s phone.
Several steps on this page are marked “(taq)” in the notebook, apparently denoting who carried them out — not resolved here to an actual name.
X-gluc application
- Defrost ✓
- Cover colonies ✓
- Leave overnight (blue colonies = β-glucosidase activity) ✓
Remaking the 40 mg/ml X-gluc stock solution: 0.025 g (25 mg) into 0.625 ml. Working solution: 20 µl of the 40 mg/ml stock mixed with 80 µl sterile water ✓. Spread over colonies ✓ — 5 µl on each colony ✓.
To do: negative control ✓; positive control ✓ on a β-glucosidase colony (from the 21 July plate) — both to be ready the next day.
Congo red application (p. 162)
Add Congo red → 15 minutes → wash with NaCl → 15 minutes → pour off → tape/parafilm the plates.