Contributors: Tank, Esther
Gel extraction (pp. 159–160)
Gel slices for the three manganese conditions selected on 4 September (0.01, 0.02, 0.05 mM MnCl₂), plus a “Without Gel” comparison column.
| 0.01 | 0.02 | 0.05 | Without Gel | |
|---|---|---|---|---|
| Gel weight | 0.255 g | 0.313 g | 0.201 g | (scribbled out) |
| Buffer | 255 µl | 313 µl | 201 µl | (scribbled out) |
| “+2x” | 510 µl | 626 µl | 402 µl | – |
An additional unlabelled row of four numbers appears above this table in the original: 1.240, 1.298, 1.186, 0.985 — not identified in the source as to what they measure.
Nanodrop results
The original is a grid with three stacked numbers per cell (conc., 260/280, 260/230, unlabelled) under three readings; several entries are scribbled out and the exact row/column alignment could not be reconstructed reliably from the transcription alone. Reproduced here in the order recorded, grouped by reading, rather than forced into a table that might misassign a number to the wrong condition.
- Reading 1: 0.01 → scribbled out, scribbled out; 0.02 → possibly 15.092 (unclear), 45.283; 0.05 → 2.302, 0.096 [?]
- Reading 2: 0.01 → 36.561, 1.838, 0.058; 0.02 → 66.128, 1.669, 0.129; 0.05 → (empty)
- Reading 3: 0.01 → 3.086, 2.287, 0.005; 0.02 → 2.922, 2.368, 0.007; 0.05 → 2.031, 2.865, 0.004
Gibson assembly of this product
- 5 µl HiFi
- 5 µl Taq product
- 0.25 µl vector PCR
- 50 °C for 15 minutes
- 2.5 µl into commercial competent cells (25 µl)
Transformation (p. 160)
- Ice, 30 minutes ✓
- 42 °C, 30 seconds ✓
- Ice, 2 minutes ✓
- 500 µl SOC, 250 rpm, 1 hour ✓ (handed off to Heather)
- Spread 50 µl + plate the rest ✓ (handed off to Heather)
- Grown overnight