Click Me Starting materials Error-prone PCR Site-Directed Mutagenesis Miniprep Asymmetric PCR Amplify Gene Amplify Backbone Amplify CsgA gene Amplify Backbone Verify Size Purify Purify Verify Size Verify Size Purify Verify Size Purify Verify Size Purify Symmetric PCR Verify Size Purify Cloning EP-PCR for CsgA Verify Size Purify Cloning Transformation 1 Inoculation in LB Plating Miniprep Pick colonies Transformation 2 Miniprep Plating Transfer colonies Assay (Day 1) Assay (Day 2) Assay (Day 3) Spectrophotometer Analyze Sequencing Analyze results

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