In order to survive in harsh anaerobic environments, certain species of Geobacter bacteria can utilize extracellular metal ions as electron acceptors for respiration, maintaining their own metabolism. This extracellular electron transfer process, in turn, relies on a specialized conductive bacterial pili to mediate it.
为了在严苛的缺氧环境中生存下去，一些种类的地杆菌可以利用细胞外的金属离子作为电子受体进行呼吸，维持自身的新陈代谢。而这种细胞外的电子传递过程需要一种特殊的导电菌毛来介导。
The conductivity of these pili is highly sensitive to the distribution of charges on its surface. This implies that such a material holds the potential to serve as a bridge linking biological systems with electronic information technology, enabling synthetic biological systems to possess both the specificity and sustainability of biological systems, as well as the precision and swiftness of electronic systems.
这种导电菌毛的电导率对其表面的电荷分布情况非常敏感，这就意味着这种材料有望作为一个连接生物系统和电子信息技术的桥梁，从而让合成生物学系统同时具有生物系统的特异性和可持续性以及电子系统的精密和快捷
This year, Tongji_China is focusing on antibody assay. Antibody assay is a crucial tool for determining the presence of specific pathogens or disease markers in human or environmental samples. In comparison to qualitative assay, quantitative antibody assay often requires a more controlled environment, more sophisticated and expensive equipment, as well as more specialized operators. Consequently, the cost and duration of each individual test are also likely to be higher.
今年Tongji_China将目光聚焦于微量抗体检测技术，抗体检测是确定人体或环境样本内是否存在特定病原体或疾病标志物的重要工具。相较于定性检测，抗体的定量检测往往需要更严格的操作环境，更复杂精密的设备，以及更专业的操作人员，对应的单次检测的价格和检测的周期也会更长。
Antibody detection techniques are commonly used in health examinations. For various reasons, auxiliary diagnostic techniques have not yet been widely used in health checkups, and primary health service centers do not have strong laboratory testing capabilities to meet the demand for comprehensive medical checkups for residents. We hope that auxiliary diagnostic techniques can promote the progress of primary health checkups in the direction of being faster, more accessible and cheaper.
在健康检查中，抗体检测项目是常用的。由于各种原因，辅助诊断技术尚未在健康检查中广泛应用，且基层卫生服务中心的实验室检查能力不强，无法满足居民全面体检需求。我们希望辅助诊断技术能促进健康检查向更快捷、更易获得、更廉价的方向进步。
This is why we have designed Plink:
a rapid,
simple, cost-effective, sensitive, and
portable real-time quantitative antibody detection system:
这就是为什么我们设计了Plink：
一种快速、简单、经济、灵敏、便携的实时定量抗体检测系统：
Innovative Design
Through our design, antibodies can selectively bind to conductive pili. This specific binding alters the charge distribution on the surface of the pili, consequently modifying their conductivity. By measuring the conductivity changes of the pili before and after binding, we can easily achieve quantitative antibody detection.
通过我们的设计，导电菌毛可以特异性结合抗体，这种特异性的结合会改变菌毛表面的电荷分布，从而改变菌毛的电导率，通过测量结合前后菌毛的电导率变化，我们就可以非常简单实现对抗体的定量检测。
Based on this, we have done the following work.
在此基础上我们做了如下工作。
1. Optimized the chassis microorganisms and provided a roadmap for future large-scale sustainable production of this material
(1)优化了底盘微生物并提供了未来大规模可持续生产这种材料的思路。
2. Devised a set of hardware and corresponding software that are compatible with our system
(2)设计了一套与我们的系统配套使用的硬件和相关软件。
3.Designed a novel model for optimizing pilus conductivity and a semi-rational directed evolution strategy
(3)设计一个全新的菌毛电导率优化模型的和半理性定向进化策略。
And this is Pilnk:
这就是Plink:
