Date	Member	Experiment	Result
2023/4/1	L.J.H. Z.S.L. Y.X.L	TSuspend the cells with complete medium. Determine the density of cells in the plate (96-well plate is usually 4000 cells/well, 100 μL/ well). Repeat in 3 wells for each group. Observe the cell density of each experimental group under the microscope. Culture in the cell incubator for 24 hours.	NK cells were infected with GOF dgRNA library.
2023/4/2	K.L.Y. Z.S.L.	Infect 5×107~1×108 cells (245 mm×245mm plate to spread cells) with the Functional MOI=0.4. Meanwhile, set up 2 parallel infection experiments as well as a blank control group (virus-free).
2023/4/6	L.J.H. K.L.Y.	Collect cells on day 14 of control group.
2023/4/7	L.J.H. Z.S.L.	Add the appropriate concentration of hygromycin B to each plate. collect the cells at least 3×107 cells.
2023/4/15	Q.X.R. K.L.Y.	The genomic DNA was extracted with the Divided Tigen Genome Extraction Kit and the remaining cells into two groups in equal amounts.
2023/4/16	L.J.H. Q.X.R. K.L.Y.	Viral supernatant were subsequently filtered with a 0.40 mm filter (Fisher / VWR) to eliminate cell debris, and finally concentrated using AmiconUltra 100 kD ultracentrifugation units (Millipore). The virus was divided into smaller portions and stored at -80 ℃.