Date	Member	Experiment	Result
2023/3/1	K.L.Y. Z.S.L.	The designed mm10dgLib was synthesized via IDT and subcloned into pLVX-EF1a-IRESPuro (Addgene, 85132).	Genome-scale GOF dgRNA library were attained.
2023/3/20	K.L.Y. Z.S.L.	mm10dgLib plasmids were obtained via Maxi preparation (Qiagen) and then comfirmed by Illumina sequencing to guarantee library representation.
2023/3/24	L.J.H. Z.S.L.	lenti-X-293 cells were seeded in 15 cm-dish at the confluency of 70-80%.	Lentivirus were produced.
2023/3/25	L.J.H. Z.S.L.	The media was substituted with 10.5 mL pre-warmed DMEM medium (Invitrogen) 2 hours before transfection. To each plate, the medium was mixed with pLVX-EF1a-IRESPuro or vector control plasmid, pCMVR8.74, pMD2.G and polybrene. Following a brief vortex, the mixture was incubated for 15 minutes at room temperature and then added drop by drop slowly to cells. 6 hours later, DMEM media was substituted with 20 mL pre-warmed DMEM media.
2023/3/27	L.J.H. Q.X.R	Gathered the viral supernatant.
2023/3/30	L.J.H. Q.X.R. K.L.Y.	Viral supernatant were subsequently filtered with a 0.40 mm filter (Fisher / VWR) to eliminate cell debris, and finally concentrated using AmiconUltra 100 kD ultracentrifugation units (Millipore). The virus was divided into smaller portions and stored at -80 ℃.