Directed evolution is widely recognized as an effective strategy for enhancing the catalytic activity of enzymes. Constructed a high-capacity mutant library and combined with high-throughput screening methods, excellent enzymes can be identified.

The optimization of metabolic engineering processes can significantly enhance the efficiency of biological production. By gene overexpressing and employing CRISPRi technology for multi-level gene interference, a high-yield substrate E. coli chassis strain was constructed.