This section provides more details on the methodology and tools used in the project alongside all troubleshooting experiments and rationale involved in experimental design.
Carbenicillin Selection System
This method was developed for high throughput wet lab screening of beneficial variants for protein refinement via directed evolution.
This method can also be used in validation of monoclonal variants when compared against the original variant.
Solubility Assay
This method was developed for a direct proxy for the solubility improvement conferred by our tags.
Reverse translation tool
Introduction to our reverse translation tool.
Protein mutator
Introduction to our protein mutator tool.
Protein analyser
The tool used to characterise protein properties based on sequence data.
Proportion optimiser
The tool used to optimise variant representation within a degenerate sequence.