Reference
[1]Cheng D, Jiang C, Xu J, et al. Characteristics and applications of alginate lyases: A review[J]. International Journal of Biological Macromolecules, 2020, 164: 1304-1320.
DOI：https://doi.org/10.1016/j.ijbiomac.2020.07.199
[2] A La Teng Zhu La, Hu YF. Advances in the preparation of alginate oligosaccharides and its biological functions. Chinese Journal of Biotechnology, 2022, 38(1): 104-118.
DOI: https://dx.doi.org/10.13345/j.cjb.210377
[3] HAN Yang, WANG Shujing.The anti-tumor effect of brown algae polysaccharide and its derivatives[J]. Chemistry of Life,2019(04):673-680.
DOI: https://dx.doi.org/10.13488/j.smhx.20180130
[4] Palackal N, Brennan Y, Callen W N, et al. An evolutionary route to xylanase process fitness[J]. Protein science, 2004, 13(2): 494-503.
DOI：https://doi.org/10.1110/ps.03333504
[5] Wang Y, Fu Z, Huang H, et al. Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge[J]. Bioresource Technology, 2012, 112(5): 275–279.
DOI：https://doi.org/10.1016/j.biortech.2012.02.092
[6] Zhang K, Liu T, Liu W, et al. Structural insights into the substrate-binding cleft of AlyF reveal the first long-chain alginate-binding mode[J]. Acta Crystallographica Section D: Structural Biology, 2021, 77(3): 336-346.
DOI：https://doi.org/10.1107/s205979832100005x
[7] Zhang Hongxiu, Liu Xiaohua, Liu Weizhi. Study on the New Alginate Lyase AlyPC1 from Marine Bacterium Pseudoalteromonas sp. 01[J].Periodical of Ocean University of China,2024,54(02):84-88+105.
DOI：http://dx.doi.org/10.16441/j.cnki.hdxb.20220128
[8]Lu Y, Zhou J, Gu Q, et al. Cloning, expression and improvement of catalytic activity of alginate lyase by site-directed mutation[J]. Systems Microbiology and Biomanufacturing, 2022, 2(3): 555-567.
DOI：https://doi.org/10.1007/s43393-022-00084-w
[9]Zhang Z, Tang L, Bao M, et al. Functional characterization of carbohydrate-binding modules in a new alginate lyase, TsAly7B, from Thalassomonas sp. LD5[J]. Marine drugs, 2019, 18(1): 25.
DOI：https://doi.org/10.3390/md18010025
[10] Li Shu,Zhang Wei,Zhao Chunmei.Directed Evolution of Alginate Lyase Alg-2 Based on Error Prone PCR[J].Food Science,2019(04):146-151.
DOI：http://dx.doi.org/10.7506/spkx1002-6630-20180207-100
[11] Qianqian Lyu, Keke Zhang, Qiaoyun Zhu, Zhijian Li, Yujie Liu, Elisabeth Fitzek, Tanner Yohe, Liming Zhao, Weihua Li, Tao Liu, Yanbin Yin, Weizhi Liu. Structural and biochemical characterization of a multidomain alginate lyase reveals a novel role of CBM32 in CAZymes, Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 9, 2018, Pages 1862-1869, ISSN 0304-4165
DOI: https://doi.org/10.1016/j.bbagen.2018.05.024
Acknowledgment
Throughout the writing of this dissertation, we have received a great deal of support and assistance. We would first like to thank our supervisor, Prof. Weizhi Liu, whose expertise was invaluable in formulating the research questions and methodology.Your insightful feedback pushed us to sharpen our thinking and brought our work to a higher level.
We would especially like to thank our advisor, Junyi Huang, who provided meticulous assistance throughout the entire process of our experiment. Without his guidance, it would not have been possible for us to complete the experiment successfully.
We would particularly like to acknowledge all of our team members, for their wonderful collaborationand persistent efforts.
We would also like to express our gratitude to WeihaoZhou as well asother graduate studentsin the lab, who provided extremely valuable guidance on the operational details of this experiment, ensuring the progress of our work.
We are grateful to our classmates, the 2024 iGEM team, OUC_China for lending us important laboratory equipment.
Finally,we also would like to express our sincere gratitude to our college--College of Marine Life Sciences, Ocean University of Chinafor providing us with the venue and experimental reagents,and encouragingus to successfully complete ourresearch. We are grateful to Professor XianghongWang forhis efforts in supplementing the experimental materials.
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