Computationally guided
evolutionary engineering
of ancestral glycosyltransferases
2024
are a large class
of biologically
active natural
products.
Among them, C-
glycosides are stable
for enzymatic or
hydrolysis.
C-Glycosides
The antioxidant
capacity of flavonoid
glycosides produces
important health
promoting activities
Flavonoid
Such as anti-aging,
anti-cancer, anti-
inflammatory,
anti-virus, anti
microorganism
and anti-diabetes
There are currently many
limitations in the chemical
synthesis of flavonoid C-
glycosides
The enzyme mediated C-
glycosylation reaction exhibits high
efficiency and regional specificity
Proved to be
the enzyme
that naturally
catalyzes
flavonoid 8C
glycosylation
reaction
High affinity,
high catalytic
activity, and
broad-spectrum
catalytic activity
towards
flavonoids
At the same time, research has
found a sucrose synthase that can
preferentially use UDP as a
substrate to produce UDPG,
providing glycosylation for
glycosylation reactions.
if we couple
the two reactions,
we will achieve higher
catalytic efficiency.
But this sucrose synthase has a higher
optional catalytic temperature, so improving
the thermal stability of TcCGT1 is of great
help for the coupling of the reaction.