Date	Member	Experiment	Result
2023/6/20	L.J.H. Q.X.R.	Target cells were plated in NK cells media in white-walled 96-well plates, followed by the addition of NK cells at E:T ratio=1:1. Finally, cytotoxicity was calculated based on LDH release using the following formula: Cytotoxicity (%) = [LDHE:T -LDHE]/LDHMax× 100%.	BBζ-M NK cells had the highest cytotoxicity.
2023/7/30	L.J.H Q.X.R.	Target cells were plated in NK cells media in white-walled 96-well plates, followed by the addition of NK cells at E:T ratio=1:1 or 5:1. Finally, cytotoxicity was calculated based on LDH release using the following formula: Cytotoxicity (%) = [LDHE:T -LDHE]/LDHMax× 100%.	BBζ-M NK cells had the highest cytotoxicity.
2023/7/20	Q.X.R. K.L.Y.	4 hours co-culture of NK cells with tumor cells, the supernatant was collected and analyzed using the quantikine ELISA human IFN-γ Immunoassay according to the manufacture’s instructions. A microplate reader (PerkinElmer, Waltham, MA, USA) was used to read the 96-well plates at 450 nm.	BBζ-M NK cells had the highest cytotoxicity.
2023/7/24	L.J.H. Z.S.L. Y.X.L.	For cell surface staining, CD107a-FITC, FLAG antibody was added and incubated for 30 min. At the completion of incubation, cells were washed with FACS buffer. For intracellular staining, cells were fixed with fixation buffer for 5 minutes on ice, following by permeabilization with perm/wash buffer overnight at 4°C. Cells were washed and stained with IFN-γ-APC, PFN-PE or GrB-APC for 30 min at 4°C, then final washed for analysis. Single cell suspensions were made in PBS (4% FBS) and washed twice in PBS 4% FBS and analyzed on an LSR Fortessa X20 (BD Biosciences).	BBζ-M NK cells had the highest cytotoxicity.