Date	Member	Experiment	Result
2023/4/25	K.L.Y.	GOF dgRNA library infected NK cells were intratumorally injected into subcutaneous AsPC-1 xenografts.	Tumor tissue were injected with infected NK cells.
2023/5/1	Q.X.R.	On day 21 after tumor inoculation, the mice were euthanized by cervical dislocation and tumor tissue was harvested. Cut tissue sample and digested by digestive solution (2.5 mg/ml type II collagenase and 10 μM Y-27632).	Tumor tissue sample were obtained.
2023/5/1	Q.X.R. K.L.Y.	Incubated tumor sample at 37℃ in the shaking incubator for thorough digestion about 1 hour. Then the digestion was filtrated by a 70 μm filter and centrifuged at 1500 rpm for 5 minutes. Centrifuge again after cells treatment and resuspend cells.	Tumor infiltrated NK cells were obtained.
2023/5/1	Z.S.L. Y.X.L.	Cells were collected and rinsed once using MACS buffer (0.5 % BSA and 2 mM EDTA in PBS). The NK cells were subsequently sorted by MojoSort™ Human NK Cell Isolation Kit (Biolegend, 480054).	NK cells were sorted.
2023/5/1	K.L.Y. Z.S.L.	Total RNA isolation, RNA quality control, library construction, sequencing and data analysis were performed by Shanghai Biotechnology Corporation (Shanghai, China). Phusion Flash High Fidelity Master Mix (Thermo Fisher) to perform two-step PCR amplification for dgRNA readout.	CRISPR screening was performed.
2023/5/2	Q.X.R. K.L.Y.	Use primers to amplify dgRNA cassette.