Date	Member	Experiment	Result
2023/9/2	L.J.H. Z.S.L. Q.X.R.	Thaw the CellTiter-Glo reagent and equilibrate to RT. Add the test compound to experimental wells, then add equivalent volume CellTiter-Glo Reagent to the cell culture medium present in each well. Agitate the mixture for 2 minutes on an orbital shaker to initiate the lysis of cells. Total luminescence signal was quantified using Living Image software.	ATP generation were analyzed.
2023/9/3	L.J.H. Z.S.L. Y.X.L.	After co-cultured with tumor cells, NK-92, BBζ cells and BBζ-M NK cells were seeded at 2 × 105 per well in cell culture microplates pre-coating with poly-lysine. Cells were equilibrated for 1 hour in XF assay medium supplemented with 10 mM glucose, 1 mM sodium pyruvate and 2 mM glutamine in a non-CO2 incubator. OCR were monitored at baseline and throughout sequential injections of oligomycin (1.5 μM), carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (0.5 μM) and rotenone or antimycin A (0.5 μM each).	Seahorse metabolic assays were performed.