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Supplementary Material

Salinity Resistance

Escherichia coli BL21 (DE3) growth curve Raw Data

Hour % NaCl in LB media (w/v) Triplicate 1 Triplicate 2 Triplicate 3 Mean Value
0 0.5 0.044 0.045 0.045 0.045
1.0 0.084 0.084 0.084 0.084
1.5 0.044 0.044 0.045 0.044
2.0 0.045 0.045 0.045 0.045
2.5 0.049 0.049 0.049 0.049
3.0 0.055 0.055 0.055 0.055
3.5 0.058 0.058 0.058 0.058
4.0 0.047 0.047 0.047 0.047
4.5 0.052 0.053 0.052 0.052
5.0 0.054 0.054 0.054 0.054
5.5 0.053 0.053 0.053 0.053
6.0 0.052 0.056 0.056 0.053
1 0.5 0.122 0.122 0.132 0.125
1.0 0.232 0.231 0.235 0.233
1.5 0.100 0.106 0.117 0.107
2.0 0.088 0.099 0.099 0.093
2.5 0.093 0.094 0.089 0.092
3.0 0.090 0.093 0.087 0.090
3.5 0.069 0.070 0.074 0.071
4.0 0.069 0.069 0.069 0.069
4.5 0.042 0.042 0.042 0.042
5.0 0.058 0.078 0.063 0.066
5.5 0.049 0.047 0.047 0.047
6.0 0.049 0.049 0.057 0.052
2 0.5 0.363 0.353 0.320 0.345
1.0 0.484 0.544 0.566 0.531
1.5 0.307 0.332 0.311 0.317
2.0 0.237 0.248 0.253 0.246
2.5 0.220 0.245 0.188 0.218
3.0 0.176 0.177 0.155 0.169
3.5 0.158 0.166 0.139 0.154
4.0 0.104 0.121 0.111 0.112
4.5 0.078 0.073 0.074 0.075
5.0 0.076 0.076 0.076 0.076
5.5 0.056 0.068 0.041 0.055
6.0 0.048 0.048 0.049 0.048
3 0.5 0.681 0.666 0.705 0.664
1.0 0.806 0.837 0.964 0.869
1.5 0.582 0.605 0.574 0.587
2.0 0.570 0.540 0.518 0.628
2.5 0.503 0.503 0.487 0.497
3.0 0.329 0.467 0.444 0.413
3.5 0.319 0.379 0.256 0.318
4.0 0.233 0.278 0.198 0.236
4.5 0.151 0.137 0.137 0.142
5.0 0.118 0.130 0.135 0.128
5.5 0.078 0.056 0.061 0.065
6.0 0.060 0.061 0.061 0.061
4 0.5 0.881 0.911 0.927 0.906
1.0 1.034 1.060 1.201 1.101
1.5 0.894 0.872 0.933 0.901
2.0 0.837 0.775 0.807 0.806
2.5 0.813 0.779 0.788 0.793
3.0 0.763 0.794 0.771 0.776
3.5 0.563 0.672 0.317 0.517
4.0 0.228 0.554 0.234 0.389
4.5 0.306 0.339 0.263 0.303
5.0 0.195 0.186 0.178 0.186
5.5 0.137 0.126 0.126 0.130
6.0 0.079 0.096 0.087 0.087
5 0.5* 0.128 0.116 0.122 1.22
1.0* 0.132 0.146 0.160 1.46
1.5* 0.104 0.103 0.111 1.060
2.0 0.997 1.022 0.952 0.990
2.5 0.934 0.923 0.963 0.990
3.0 1.015 1.027 0.963 1.001
3.5 0.584 0.585 0.309 0.492
4.0 0.159 0.648 0.122 0.309
4.5 0.205 0.456 0.303 0.321
5.0 0.177 0.200 0.261 0.212
5.5 0.245 0.236 0.227 0.236
6.0 0.110 0.129 0.106 0.115
6 0.5* 0.105 0.128 0.109 1.14
1.0* 0.168 0.168 0.178 1.713
1.5* 0.129 0.137 0.122 1.293
2.0 1.148 1.102 1.113 1.121
2.5 1.130 1.160 1.146 1.145
3.0 1.131 1.073 1.032 1.074
3.5 0.834 0.893 0.596 0.724
4.0 0.223 0.735 0.096 0.357
4.5 0.203 0.607 0.107 0.305
5.0 0.197 0.133 0.149 0.159
5.5 0.387 0.348 0.331 0.355
6.0 0.189 0.240 0.179 0.202

Table 1) Escherichia coli BL21 (DE3) growth curve in various salt concentrations in LB. E.coli BL21 were inoculated from overnight culture in triplicate into LB media with various salt concentrations. These were then incubated at 250rpm in 37°C and samples were taken and the OD600 measured every 1 hour. Cultures with an OD600 significantly above 1 were diluted 10 fold and their mean value calculated by multiplying by 10, these are indicated by .*

Sequences

Part Name Sequence
stpa Gene Block sequence CAGTGAAGACATTGCCGGAGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACATACTAGAGAAAGAGGAGAAATACTAAATGGTATTACACCAACAACGTTTCTCCCTCGACCATGGAGCTTTTTGTCAAACCTTAGCCCAAACTGAAAATTTACTCATTGTCCAAGACTTGGATGGGGTCTGCATGGAATTAGTGCAAGATCCCCTCAGTCGCCGCCTGGATGCCGATTATGTCCGGGCCACCACCCTGTTTGCTGAACATTTTTACGTGTTGACCAATGGGGAGCACGTGGGAAAAAGAGGAGTACAGGGCATTGTGGAACAATCCTTTGGGGATGCTTCCTTTGTGCAACAGGAAGGCCTATATTTGCCCGGTTTGGCGGCCGGGGGAGTGCAGTGGCAGGATCGCCATGGCAAAGTAAGTCATCCTGGAGTGGGGCAAACGGAGCTGGAGTTTTTAGCGGCGGTGCCCGAAAAAATCACTAATTGTTTAAAAACCTTTTTTGGCGATCGCCCCCATTCCCTATCCCCAGAGCAATTACAAACGGGCATTGAAGCTTCGGTTTTAGATAATGTGGCTTCCCCCACCGCCAATTTAAATACCTTGGCCAATCTGTTACAAGACTTTCCGCAAATTTACCGAGATTTGCAGGAAACCATGGCTCAATTATTGGATCAGTTGATGGCGGAAGCCGTTGCCCAGGGTTTGGGGAATAGTTTTTTTGTCCACTATGCTCCCAATTTAGGTAGGGATGAACGAGGTAAGGAAATTATTCGTTGGGCCAAAGCTGGGGATTCCGGCACCACCGATTTTCAATTTATGTTGCGGGGTGGGGTCAAAGAAGCCGGGGTTTTGGCTTTGCTAAATCGTTACTATCACAATCGGACAGGGCAATATCCTCTGGGAGAAAGTTTTAGTGCTCGCCAAGCGCCCCCATCCCACCAGGACTTGTTGCATTTGGTGAAAGCGCAATTTGATCCGGCCTTGATGCCGCTGATCATTGGAGTTGGGGATACGGTCACCAGTCAGGTGGATGAAGCTACCGGGGAAATTCGACGTGGCGGGAGCGATCGCCAATTTTTGCAATTAATCCAAGATTTGGGGGATTGGGGAAATCACGGTAACTTAGTGGTGTATGTGGACAGTTCCCAGGGGGAGGTGAAAAATCGCCAACCTCTACAACTAGAAACCGTGGCGGGGCAAACCCAAGTGGTGGCTGGCCCTGGGGATATGCGGGACAGGGAAGAGCCATTGAAGATCAATGTGGCTTTTCCTGGTGGCCATGACCAATATGTAGCGGCGTTTAAGCAGGCGGCCCAGCGCCGAAGAGTCCATTTTTCCCAGTAGTTATGGTGGGCTTCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGCGCTGCAATGAGACCGAGGATGCACATGTGACCGAGGGATAGTCTTCTGAC

Shows the sequence of the stpa gene block. Overhangs are in italics. Recognition sites are in bold

PHB

Sequences

Part Name Sequence
Hybrid Promotor phaCAB Assembly aatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagactagtggaagacatGGAGttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagaggattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccggactggacagcctggctggcgggccaagcaggcgcaaaacgcgctgcgccagccaactatggcaacgcgcgctaccgcgcgattgaacccgcgcctggccgctatgtaaaagcaaaagcgtgacgcttgcatgagtgccggcgtgcgtcatgcacggcgccggcaggcctgcacgttccctcccgtttccattgaaaggactacacaatgaccgatgttgttatcgtcagtgccgcgcgcaccgccgtgggtaaatttggcggcagtctggcgaaaatcccggcgccagaattgggcgcggtggtaatcaaagcggccctggaacgcgccggtgtgaaacctgagcaggtgagcgaagtaattatgggtcaggttctgaccgcgggcagcgggcaaaatccggcgcgtcaggctgcgattaaggcaggccttccggcgatggtgcccgcgatgaccattaacaaagtatgcggatcgggactgaaagcagtgatgctggccgctaacgccattatggcaggcgacgcggaaattgtggtcgctggcggacaggagaatatgtcagccgcgcctcatgtgctgccgggtagccgcgacggctttcgcatgggtgatgcaaaactggtcgatactatgattgttgacggcttatgggatgtgtacaaccagtaccacatgggcatcaccgccgagaacgtggccaaggaatacggcatcacacgcgaggcgcaggatgagttcgccgtcggctcgcagaacaaggccgaagccgcgcagaaggccggcatgtttgacgaagagatcgtcccggtgctgatcccgcagcgcaagggcgacccggtggccttcaagaccgacgagttcgtgcgccagggcgccacgctggacagcatgtccggcctcaagcccgccttcgacaaggccggcacggtgaccgcggccaacgcctcgggcctgaacgacggcgccgccgcggtggtggtgatgtcggcggccaaggccaaggaactgggcctgaccccgctggccacgatcaagagctatgccaacgccggtgtcgatcccaaggtgatgggcatgggcccggtgccggcctccaagcgcgccctgtcgcgcgccgagtggaccccgcaagacctggacctgatggagatcaacgaggcctttgccgcgcaggcgctggcggtgcaccagcagatgggctgggacacctccaaggtcaatgtgaacggcggcgccatcgccatcggccacccgatcggcgcgtcgggctgccgtatcctggtgacgctgctgcacgagatgaagcgccgtgacgcgaagaagggcctggcctcgctgtgcatcggcggcggcatgggcgtggcgctggcagtcgagcgcaaataaggaaggggttttccggggccgcgcgcggttggcgcggacccggcgacgataacgaagccaatcaaggagtggacatgactcagcgcattgcgtatgtgaccggcggcatgggtggtatcggaaccgccatttgccagcggctggccaaggatggctttcgtgtggtggccggttgcggccccaactcgccgcgccgcgaaaagtggctggagcagcagaaggccctgggcttcgatttcattgcctcggaaggcaatgtggctgactgggactcgaccaagaccgcattcgacaaggtcaagtccgaggtcggcgaggttgatgtgctgatcaacaacgccggtatcacccgcgacgtggtgttccgcaagatgacccgcgccgactgggatgcggtgatcgacaccaacctgacctcgctgttcaacgtcaccaagcaggtgatcgacggcatggccgaccgtggctggggccgcatcgtcaacatctcgtcggtgaacgggcagaagggccagttcggccagaccaactactccaccgccaaggccggcctgcatggcttcaccatggcactggcgcaggaagtggcgaccaagggcgtgaccgtcaacacggtGtctccgggctatatcgccaccgacatggtcaaggcgatccgccaggacgtgctcgacaagatcgtcgcgacgatcccggtcaagcgcctgggcctgccggaagagatcgcctcgatctgcgcctggttgtcgtcggaggagtccggtttctcgaccggcgccgacttctcgctcaacggcggcctgcatatgggctgacctgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataGCTTatgtcttctactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaa
BBa_K1149051 (IDT G-Block) GTCAggtctcAGGAGttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagaggattcccgcattgacagcgcgtgcgttgcaaggcaacaatggactcaaatgtctcggaatcgctgacgattcccaggtttctccggcaagcatagcgcatggcgtctccatgcgagaatgtcgcgcttgccggataaaaggggagccgctatcggaatggacgcaagccacggccgcagcaggtgcggtcgagggcttccagccagttccagggcagatgtgccggcagaccctcccgctttgggggaggcgcaagccgggtccattcggatagcatctccccatgcaaagtgccggccagggcaatgcccggagccggttcgaatagtgacggcagagagacaatcaaatcatggctactgggaaaggagccgccgccagcacccaggaaggcaaaagccaaccatttaaagtgaccccaggcccgttcgatcctgccacctggctggaatggtcacgtcaatggcaaggtacggagggcaacggccatgcggcagcgtccggcatccctgggcttgatgcactggctggtgttaaaattgcgcctgcccaattgggagacatccaacagcgttatatgaaagatttttcggcgctgtggcaggcgatggcggaaggtaaagcagaggcgacgggaccgctgcatgaccgtcgctttgctggcgacgcctggcgtaccaatttgccctatcgttttgcggcagccttctacctgctgaacgctcgtgccctgactgagctggcagatgctgtcgaagcggatgccaaaacccgccaacgcatccgttttgccatctcacagtgggtcgacgcaatgtcaccggccaacttcctggccaccaatccggaagcacagcgcctcctgattgaaagcggcggggaatcgctgcgcgccggcgttcgtaacatgatggaagatctgacccgtggtaagatttcgcagacggatgagtcggccttcgaggtgggtcgcaatgttgcagtgacagaaggcgcggtggtgtttgagaacgaatactttcaacttctccagtataaaccactgacggataaagtgcatgcgcgcccattgttaatggttcccccgtgcattaataaatactatatcctggatcttcagccggaaagctccttagtgcgccacgtggtggaacaggggcatactgtgtttttagttagctggcgcaatccagatgcctcaatggccggcagtacctgggatgactacattgaacacgcggctattcgcgcgatcgaagtggcacgcgacatctcaggtcaggacaagattaacgtactcggcttttgcgtcggtggcactatcgtgagcaccgctctggcggtactggctgcgcgtggtgaacaccccgcagcgtcggttacgcttctgaccacactgttggatttcgccgacactggcatcttggatgtatttgtcgatgagggccacgttcagctgcgcgaggccactctgggtggcggcgccggcgcaccgtgcgctctgctgcgcggtctggagctcgcgaatactttttctttcttacgcccgaacgatcttgtctggaattacgtagttgacaactatctcaaaggtaacacgcccgtgccgtttgatttattattctggaatggggatgcgaccaacctgccgggtccttggtattgttggtatctccgtcatacatacttacagaatgaattaaaggtcccaggtaaacttactgtgtgtggtgtcccggtagatctggcgagtatcgacgtgccgacctacatttatgggagtcgtgaagatcatattgtgccgtggaccgccgcgtacgcttctaccgcgcttctggcaaacaaattacgtttcgttctgggtgccagcggccacattgcgggtgttattaatcctccggccaaaaataaacgctcgcattggacaaacgacgccttgccggagagccctcagcagtggttggcgggtgccattgagcaccatggaagttggtggccggactggacagcctggctggcgggccaagcaggcgcaaaacgcgctgcgccagccaactatggcaacgcgcgctaccgcgcgattgaacccgcgcctggccgctatgtaaaagcaaaagcgtgacgcttgcatgagtgccggcgtgcgtcatgcacggcgccggcaggcctgcacgttccctcccgtttccattgaaaggactacacaatgaccgatgttgttatcgtcagtgccgcgcgcaccgccgtgggtaaatttggcggcagtctggcgaaaatcccggcgccagaattgggcgcggtggtaatcaaagcggccctggaacgcgccggtgtgaaacctgagcaggtgagcgaagtaattatgggtcaggttctgaccgcgggcagcgggcaaaatccggcgcgtcaggctgcgattaaggcaggccttccggcgatggtgcccgcgatgaccattaacaaagtatgcggatcgggactgaaagcagtgatgctggccgctaacgccattatggcaggcgacgcggaaattgtggtcgctggcggacaggagaatatgtcagccgcgcctcatgtgctgccgggtagccgcgacggctttcgcatgggtgatgcaaaactggtcgatactatgattgttgacggcttatgggatgtgtacaaccagtaccacatgggcatcaccgccgagaacgtggccaaggaatacggcatcacacgcgaggcgcaggatgagttcgccgtcggctcgcagaacaaggccgaagccgcgcagaaggccggcatgtttgacgaagagatcgtcccggtgctgatcccgcagcgcaagggcgacccggtggccttcaagaccgacgagttcgtgcgccagggcgccacgctggacagcatgtccggcctcaagcccgccttcgacaaggccggcacggtgaccgcggccaacgcctcgggcctgaacgacggcgccgccgcggtggtggtgatgtcggcggccaaggccaaggaactgggcctgaccccgctggccacgatcaagagctatgccaacgccggtgtcgatcccaaggtgatgggcatgggcccggtgccggcctccaagcgcgccctgtcgcgcgccgagtggaccccgcaagacctggacctgatggagatcaacgaggcctttgccgcgcaggcgctggcggtgcaccagcagatgggctgggacacctccaaggtcaatgtgaacggcggcgccatcgccatcggccacccgatcggcgcgtcgggctgccgtatcctggtgacgctgctgcacgagatgaagcgccgtgacgcgaagaagggcctggcctcgctgtgcatcggcggcggcatgggcgtggcgctggcagtcgagcgcaaataaggaaggggttttccggggccgcgcgcggttggcgcggacccggcgacgataacgaagccaatcaaggagtggacatgactcagcgcattgcgtatgtgaccggcggcatgggtggtatcggaaccgccatttgccagcggctggccaaggatggctttcgtgtggtggccggttgcggccccaactcgccgcgccgcgaaaagtggctggagcagcagaaggccctgggcttcgatttcattgcctcggaaggcaatgtggctgactgggactcgaccaagaccgcattcgacaaggtcaagtccgaggtcggcgaggttgatgtgctgatcaacaacgccggtatcacccgcgacgtggtgttccgcaagatgacccgcgccgactgggatgcggtgatcgacaccaacctgacctcgctgttcaacgtcaccaagcaggtgatcgacggcatggccgaccgtggctggggccgcatcgtcaacatctcgtcggtgaacgggcagaagggccagttcggccagaccaactactccaccgccaaggccggcctgcatggcttcaccatggcactggcgcaggaagtggcgaccaagggcgtgaccgtcaacacggtGtctccgggctatatcgccaccgacatggtcaaggcgatccgccaggacgtgctcgacaagatcgtcgcgacgatcccggtcaagcgcctgggcctgccggaagagatcgcctcgatctgcgcctggttgtcgtcggaggagtccggtttctcgaccggcgccgacttctcgctcaacggcggcctgcatatgggctgacctgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataGCTTcgagaccGACG
DVK_AE aatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagactagtggaagacatGGAGagagacctgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcgggggtctctGCTTatgtcttctactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaa