iDEC 2022 | OUC_China

Directed evolution of carbonic anhydrase to improve microbial induced calcium carbonate precipitation (MICP) efficienc

Microbial induced calcium carbonate precipitation (MICP) is an environmentally friendly way to produce inorganic material, and it`s considered as a feasible approach to resolve pollution problem and incompatible surface of inorganic and organic materials in repairation of concrete and soil. Urease and carbonic anhydrase are two key enzymes in MICP, which cooperate in the process. We express carbonic anhydrase in Escherichia coli and modify it by error-prone PCR, which is a widely used directed evolution technique. We obtain several mutants and measure their enzyme activity successfully. The structure of these mutants was predicted and analysed by means of bioinformatics.