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These are all the necessary files employed in team CPU_CHINA in iDEC 2022.The related data used to support the findings of this study have been deposited in the Open Science Foundation repository (DOI:10.17605/OSF.IO/9NBSJ); other related data are available from the corresponding author upon request

Data Accessibility

The file includes experimental protocols applied inside our lab.

CPU_CHINA iDec experiment records.md

The sequence data used to support the findings of this study have been deposited in the NCBI database(PDB ID:7LYT_A, 7LYT).

    
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DNA sequence.fa
Cas Φ(Wild-type) sequence.fa
nCasΦ sequence.fa
vCasΦ sequence.fa
CasΦ-GSSG5 sequence.fa
CasΦ-Neg K sequence.fa
CasΦ-POS E Sequence.fa
CasΦ-POS Neg Sequence.fa

The CrRNA sequence data used to support the findings of this study have been deposited in the NCBI database(SUB12134155,SUB12134154,SUB12134152,SUB12134151,SUB12134149,SUB12134148,SUB12134147,SUB12134145,SUB12134122,SUB12134108,SUB12134100,SUB12134094). The data used to support the findings of this study are included within the article.

    

    
        

            
            
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The reaction rates of FQ reporter cleavage by Cas-crRNA in the presence of DNA targets with different concentrations.xlsx
The reaction rates of FQ reporter cleavage by Cas-crRNA in the presence of DNA targets with single-base mismatch.xlsx
The reaction rates of FQ reporter cleavage by Mut-4 with hairpin structure crRNA.xlsx
The reaction rates of FQ reporter cleavage with samples containing 50% to 0% DNA mutations.xlsx
The time-course fluorescence intensity curves of FQ reporter cleavage by different Cas-crRNA in the presence of DNA targets.xlsx